Gene therapy is seen as an approach to curing diseases by delivering therapeutic genes into the cell to repair incorrectly coded genes or by introducing a new gene to alter cell function. Gene delivery has received a lot of attention in recent decades due to its various applications in biomedical sciences, such as diagnostic devices, tissue regeneration, biosensors, cancer therapy, and vaccine development. Common techniques include electroporation, microinjection, ultrasonic and magnetic transfection, as well as viral vectors and non-viral vectors. The development of nanotechnology has led to the construction of non-toxic delivery systems with low toxicity and high transfection efficiency, such as lipids and polymers. Surface-mediated gene transport appears to play a crucial role in gene delivery due to various physicochemical properties and good biocompatibility. Using the BiomACS screening platform, we have found that topography appears to play a role in improving the transfection of stem cells, such as human bone marrow mesenchymal stem cells (hBM-MSCs) and myoblast cells, which is promising for non-viral gene transfer and offers alternative approaches to improve cell culture and gene therapies.
Publicaties:
- L. Ge, L. Yang, R. Bron, P. van Rijn, ” Topography-Mediated Enhancement of Nonviral Gene Delivery in Stem Cells “, Pharmaceutics, 14(5), 1096. https://doi.org/10.3390/pharmaceutics14051096